PHARMACOGNOSTIC AND ANTICONVULSANT STUDIES ON GLOSSONEMA BOVEANUM DECNE (APOCYNACEAE)
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
Glossonema boveanum Decne is a hairy canescent perennial herb belonging to the Apocynaceae. Traditionally, the powdered whole plant is use for the treatment of many illnesses including epilepsy. This study is aimed at establishing some of the pharmacognostic features of the powdered whole plant; investigating the phytochemical compounds of the plant and evaluating the anticonvulsant property of its aqueous ethanol extract. The powdered whole plant material was examined by microscopy and its physicochemical parameters such as: Moisture content, extractive values and ash values were also determined. Thin layer chromatographic technique was used for the phytochemical screening of the various fractions of the aqueous ethanol extract of the plant. Using silica gel column chromatography and preparative thin layer chromatography, a more detailed phytochemical screening was carried out on the n-Hexane fraction of the extract. Maximal electroshock-induced seizure model in chicks and Pentylenetetrazole (PTZ)-induced seizure model in mice were used for the anticonvulsant studies. The intra-peritoneal median lethal dose (LD50) of the extract on mice and chicks was also determined. Microscopical examination of the powdered whole plant of G. boveanum identified the presence of polygonal epidermal cells, anisocytic stomatal cells, covering trichomes with cystolith incrusted in them, Starch grains, suberins, lignins and aleurone grains were also found to be present. Thin layer chromatographic analysis on the various fractions of aqueous ethanol extract revealed the presence of phenolic compounds, triterpenoids, flavonoids, and anthracene derivatives. Column chromatography and Preparative thin layer chromatography carried out on the n-Hexane fraction of the extract led to the isolation of two pentacyclic triterpenoids; Compound SAL1 and compound SAL2 which were later identified using NMR spectroscopic analysis as α-amyrin acetate and lupeol respectively. At the highest dose used (140 mg/kg), the extract conferred 40% protection against maximal electroshock induce seizure in chicks with no significant decrease in viii recovery period against it. Phenytoin (20 mg/kg), the standard anticonvulsant employed conferred 90% protection against maximal electroshock-induced seizure. The extract did not exhibit any significant effect against pentelenetetrazole induced seizure in mice. The intraperitonial median lethal dose of the extract on mice and chicks was determined to be 1246 mg/kg and 471 mg/kg respectively. All these findings on G. boveanum are being reported for the first time. The result suggests that the aqueous ethanol extract of the plant contains pharmacologically active compound(s) that may be valuable in management of generalized tonic-clonic and or partial seizures
Related Project Materials
ANGLICANISM AND THE CHALLENGES OF SEXUAL ABERRATIONS- THE RESPONSE OF THE CHURCH OF NIGERIA
Background to the Study
Religion has always been the main motivator of morality from the inception of the human society...
AN EMPIRICAL ANALYSIS OF THE IMPACT OF PRIVATE SECTOR ON THE ECONOMIC GROWTH AND DEVELOPMENT OF NIGERIA (1980–2010)
ABSTRACT
The study examines the private sector as the engine of economic growth and development in Nigeria. A model was specified and dat...
EFFECT OF WORKING CAPITAL ON THE OPERATION OF BUSINESS ORGANIZATION
ABSTRACT
This research work is to know the effect of working capital on the operation...
EFFECT OF COVID-19 ON THE BANKING SECTOR
BACKGROUND OF STUDY
The COVID-19 outbreak is a global pandemic. It originated in December 2019 in the C...
EVALUATION ON TAXATION AS A MOTIVATION FOR GROWTH AND DEVELOPMENT IN NIGERIA
Background of the study
Responsible governments worldwide, whether at the federal, state, or local levels, are concerned...
ECONOMIC ANALYSIS OF PINEAPPLE PRODUCTION
Background to the Study
Nigeri...
NIGERIAN WORKERS ATTITUDE TO WORK AND ITS EFFECT ON PRODUCTIVITY
ABSTRACT
This study was carried out to examine Nigerian workers attitude to work and its effect on...
THE EFFECT OF RATIO ANALYSIS IN INVESTMENT DECISION
ABSTRACT
This study dwells on The Effect of Ratio Analysis in Investment Decision in First Bank Nigeria Plc. The study b...
IMPACT OF LANGUAGE BARRIERS ON ADULT EDUCATION PARTICIPATION
Abstract: The research investigates the impact of language barriers on adult education participation. This study aimed to explore how language...
INFLUENCE OF MENTORING AND ROLE MODELING IN PROMOTING SENIOR SECONDARY SCHOOL GIRLS ATTITUDES AND SELF–EFFICACY IN CHEMISTRY
Abstract
This study was carried out to identify senior secondary school girls’ mentors and role m...
